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x16 slot is the most capable slot of those and if you install an x16 supported card it works at fastest x8, x4 and x1 slots are

I tried plugging one of my PCIe x1 cards into the bottom PCIe x16 slot, and the motherboard didn't read it at all I then plugged it into the Yes, a x1 card should work in a x16 slot It's good that you've got it to work in a x1 slot What else have you tried it in besides the x16 slot

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